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receptor-destroying enzyme (rde (ii) “seiken  (Denka Co Ltd)

 
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    Structured Review

    Denka Co Ltd receptor-destroying enzyme (rde (ii) “seiken
    Receptor Destroying Enzyme (Rde (Ii) “Seiken, supplied by Denka Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/receptor+destroying+enzyme+(rde)/receptor+destroying+enzyme++rde/pm40573959-101-14-15
    Average 90 stars, based on 1 article reviews
    receptor-destroying enzyme (rde (ii) “seiken - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Human COBRA 2 vaccine contains two major epitopes that are responsible for eliciting neutralizing antibody responses against heterologous clades of viruses.
    Article Snippet: Highly pathogenic H5N1 influenza viruses continue to spread around the globe and reassort with low pathogenic avian influenza viruses often resulting in morbidity and mortality to not only waterfowl, but also poultry.. Our group previously developed two hemagglutinin (HA) based vaccines using a methodology termed computationally optimized broadly reactive antigen (COBRA).. Each of these HA antigens, Human COBRA 2 (Hu-CO) and Human-Avian COBRA 2 (Hu-Av CO) elicit antibodies with hemagglutination-inhibition (HAI) activity against viruses from various clades, but not always the same viruses.

    Article Title: Prior infection with classical swine H1N1 influenza viruses is associated with protective immunity to the 2009 pandemic H1N1 virus
    Article Snippet: Sera were treated with 3:1 (v/v) receptor destroying enzyme (RDE; Denka‐Seiken Co., Nihonbashi, Japan), incubated at 37°C for 14–20 hours, heated at 56°C for 30 minutes, allowed to cool, and diluted 1:10 in PBS.

    Article Title: Exploring heterologous prime-boost vaccination approaches to enhance influenza control in pigs
    Article Snippet: Before the HI assay, the serum was separated from the blood cells and the serum was treated with three parts receptor destroying enzyme (RDE; Denka Seiken, Tokyo, Japan) for 18 h at 37 °C, followed by heat inactivation at 56 °C for 30 min. After the sera cooled down to room temperature, 20% of turkey red blood cells were added and hemadsorbed for at least 30 min to remove nonspecific hemagglutinin inhibitors and natural serum agglutinins.

    Article Title: Molecular-level analysis of the serum antibody repertoire in young adults before and after seasonal influenza vaccination
    Article Snippet: Antibody binding was allowed to proceed for 300 s and normalized to a control antibody (5J8 for RBD, 39.29 for HA stem and full-length HA).

    Article Title: Intranasal vaccination with a replication-deficient influenza virus induces heterosubtypic neutralising mucosal IgA antibodies in humans.
    Article Snippet: We investigated the cross-neutralising potential of serum and nasal wash samples from volunteers who were intranasally immunised once with a monovalent replication-deficient delNS1-H1N1 influenza virus vaccine (7.7 log10 TCID50/volunteer).. Eight out of twelve (8/12) vaccinees responded to vaccination with a significant increase of antibody levels in serum IgG ELISA, mucosal IgA ELISA, MNA or HAI.. Four responders showed delNS1-specific ELISA IgA increases and revealed excellent homosubtypic neutralising activity in serum and mucosal washings (4/4).

    Article Title: Human Monoclonal Antibody Derived from Transchromosomic Cattle Neutralizes Multiple H1 Clades of Influenza A Virus by Recognizing a Novel Conformational Epitope in the Hemagglutinin Head Domain
    Article Snippet: The mAb 53C10 was treated with Receptor-228 Destroying Enzyme (RDE, Denka Seiken) at 37°C for 18 hours, heat-inactivated at 56°C, and 229 two-fold serial-diluted in PBS in a U-bottom 96 well plate (Thermo Fisher Scientific).

    Hemagglutination Assay:

    Article Title: A boosting skin vaccination with dissolving microneedle patch encapsulating M2e vaccine broadens the protective efficacy of conventional influenza vaccines A novel prime-boost influenza vaccination stratagem
    Article Snippet: .. In brief, sera were treated with receptor destroying enzyme (RDE, Denka Seiken; Tokyo, Japan) overnight at 37°C followed by 30 min at 56°C Treated samples were serially two-fold diluted and mixed with 4 HA units/25 μl of Aichi or PR8 virus for 15 min. 50 μl of 0.5% Turkey red blood cells (Lampire Biological Laboratories) were added and agglutination was observed after 30 min. ..

    Virus:

    Article Title: A boosting skin vaccination with dissolving microneedle patch encapsulating M2e vaccine broadens the protective efficacy of conventional influenza vaccines A novel prime-boost influenza vaccination stratagem
    Article Snippet: .. In brief, sera were treated with receptor destroying enzyme (RDE, Denka Seiken; Tokyo, Japan) overnight at 37°C followed by 30 min at 56°C Treated samples were serially two-fold diluted and mixed with 4 HA units/25 μl of Aichi or PR8 virus for 15 min. 50 μl of 0.5% Turkey red blood cells (Lampire Biological Laboratories) were added and agglutination was observed after 30 min. ..

    Agglutination:

    Article Title: A boosting skin vaccination with dissolving microneedle patch encapsulating M2e vaccine broadens the protective efficacy of conventional influenza vaccines A novel prime-boost influenza vaccination stratagem
    Article Snippet: .. In brief, sera were treated with receptor destroying enzyme (RDE, Denka Seiken; Tokyo, Japan) overnight at 37°C followed by 30 min at 56°C Treated samples were serially two-fold diluted and mixed with 4 HA units/25 μl of Aichi or PR8 virus for 15 min. 50 μl of 0.5% Turkey red blood cells (Lampire Biological Laboratories) were added and agglutination was observed after 30 min. ..



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    Journal: Cell Reports Medicine

    Article Title: Modulation of lipid nanoparticle-formulated plasmid DNA drives innate immune activation promoting adaptive immunity

    doi: 10.1016/j.xcrm.2025.102035

    Figure Lengend Snippet:

    Article Snippet: Receptor destroying enzyme (RDE) , Hardy Diagnostics , Cat# 370013.

    Techniques: Virus, Recombinant, Lysis, Reporter Gene Assay, Cell Stimulation, Electron Microscopy, Luminex, Enzyme-linked Immunospot, Luciferase, Plasmid Preparation, Software, Synthesized